LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community.
Identifieur interne : 000064 ( Main/Exploration ); précédent : 000063; suivant : 000065LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community.
Auteurs : Taylor J. Moehling [États-Unis] ; Gihoon Choi [États-Unis] ; Lawrence C. Dugan [États-Unis] ; Marc Salit [États-Unis] ; Robert J. Meagher [États-Unis]Source :
- Expert review of molecular diagnostics [ 1744-8352 ] ; 2021.
Abstract
Introduction: Over the past decade, loop-mediated isothermal amplification (LAMP) technology has played an important role in molecular diagnostics. Amongst numerous nucleic acid amplification assays, LAMP stands out in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions. Areas covered: In this review, we outline the front-end LAMP design practices for point-of-care (POC) applications, including sample handling and various signal readout methodologies. Next, we explore existing LAMP technologies that have been validated with clinical samples in the field. We summarize recent work that utilizes reverse transcription (RT) LAMP to rapidly detect SARS-CoV-2 as an alternative to standard PCR protocols. Finally, we describe challenges in translating LAMP from the benchtop to the field and opportunities for future LAMP assay development and performance reporting. Expert opinion: Despite the popularity of LAMP in the academic research community and a recent surge in interest in LAMP due to the COVID-19 pandemic, there are numerous areas for improvement in the fundamental understanding of LAMP, which are needed to elevate the field of LAMP assay development and characterization.
DOI: 10.1080/14737159.2021.1873769
PubMed: 33474990
Affiliations:
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Meagher</name><affiliation wicri:level="2"><nlm:affiliation>Sandia National Laboratories, Biotechnology & Bioengineering Dept ., Livermore, CA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Sandia National Laboratories, Biotechnology & Bioengineering Dept ., Livermore, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Expert review of molecular diagnostics</title><idno type="eISSN">1744-8352</idno><imprint><date when="2021" type="published">2021</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><b>Introduction</b>: Over the past decade, loop-mediated isothermal amplification (LAMP) technology has played an important role in molecular diagnostics. Amongst numerous nucleic acid amplification assays, LAMP stands out in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions. <b>Areas covered</b>: In this review, we outline the front-end LAMP design practices for point-of-care (POC) applications, including sample handling and various signal readout methodologies. Next, we explore existing LAMP technologies that have been validated with clinical samples in the field. We summarize recent work that utilizes reverse transcription (RT) LAMP to rapidly detect SARS-CoV-2 as an alternative to standard PCR protocols. Finally, we describe challenges in translating LAMP from the benchtop to the field and opportunities for future LAMP assay development and performance reporting. <b>Expert opinion</b>: Despite the popularity of LAMP in the academic research community and a recent surge in interest in LAMP due to the COVID-19 pandemic, there are numerous areas for improvement in the fundamental understanding of LAMP, which are needed to elevate the field of LAMP assay development and characterization.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33474990</PMID><DateRevised><Year>2021</Year><Month>01</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1744-8352</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2021</Year><Month>Jan</Month><Day>27</Day></PubDate></JournalIssue><Title>Expert review of molecular diagnostics</Title><ISOAbbreviation>Expert Rev Mol Diagn</ISOAbbreviation></Journal><ArticleTitle>LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community.</ArticleTitle><Pagination><MedlinePgn>1-19</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/14737159.2021.1873769</ELocationID><Abstract><AbstractText><b>Introduction</b>: Over the past decade, loop-mediated isothermal amplification (LAMP) technology has played an important role in molecular diagnostics. Amongst numerous nucleic acid amplification assays, LAMP stands out in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions. <b>Areas covered</b>: In this review, we outline the front-end LAMP design practices for point-of-care (POC) applications, including sample handling and various signal readout methodologies. Next, we explore existing LAMP technologies that have been validated with clinical samples in the field. We summarize recent work that utilizes reverse transcription (RT) LAMP to rapidly detect SARS-CoV-2 as an alternative to standard PCR protocols. Finally, we describe challenges in translating LAMP from the benchtop to the field and opportunities for future LAMP assay development and performance reporting. <b>Expert opinion</b>: Despite the popularity of LAMP in the academic research community and a recent surge in interest in LAMP due to the COVID-19 pandemic, there are numerous areas for improvement in the fundamental understanding of LAMP, which are needed to elevate the field of LAMP assay development and characterization.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Moehling</LastName><ForeName>Taylor J</ForeName><Initials>TJ</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-1848-7078</Identifier><AffiliationInfo><Affiliation>Sandia National Laboratories, Biotechnology & Bioengineering Dept ., Livermore, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Gihoon</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Sandia National Laboratories, Biotechnology & Bioengineering Dept ., Livermore, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dugan</LastName><ForeName>Lawrence C</ForeName><Initials>LC</Initials><AffiliationInfo><Affiliation>Lawrence Livermore National Laboratory, Biosciences & Biotechnology Div ., Livermore, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Salit</LastName><ForeName>Marc</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Joint Initiative for Metrology in Biology, SLAC National Accelerator Lab and Departments of Bioengineering and Pathology, Stanford University , Stanford, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meagher</LastName><ForeName>Robert J</ForeName><Initials>RJ</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-6262-2126</Identifier><AffiliationInfo><Affiliation>Sandia National Laboratories, Biotechnology & Bioengineering Dept ., Livermore, CA, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2021</Year><Month>01</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Expert Rev Mol Diagn</MedlineTA><NlmUniqueID>101120777</NlmUniqueID><ISSNLinking>1473-7159</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Loop-mediated isothermal amplification</Keyword><Keyword MajorTopicYN="N">SARS-CoV-2</Keyword><Keyword MajorTopicYN="N">diagnostics</Keyword><Keyword MajorTopicYN="N">point-of-care</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2021</Year><Month>1</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2021</Year><Month>1</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2021</Year><Month>1</Month><Day>21</Day><Hour>8</Hour><Minute>37</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33474990</ArticleId><ArticleId IdType="doi">10.1080/14737159.2021.1873769</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Moehling, Taylor J" sort="Moehling, Taylor J" uniqKey="Moehling T" first="Taylor J" last="Moehling">Taylor J. Moehling</name></region><name sortKey="Choi, Gihoon" sort="Choi, Gihoon" uniqKey="Choi G" first="Gihoon" last="Choi">Gihoon Choi</name><name sortKey="Dugan, Lawrence C" sort="Dugan, Lawrence C" uniqKey="Dugan L" first="Lawrence C" last="Dugan">Lawrence C. Dugan</name><name sortKey="Meagher, Robert J" sort="Meagher, Robert J" uniqKey="Meagher R" first="Robert J" last="Meagher">Robert J. Meagher</name><name sortKey="Salit, Marc" sort="Salit, Marc" uniqKey="Salit M" first="Marc" last="Salit">Marc Salit</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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